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Journal / Bioengineering / Fabricação e Uso de Andaimes Secos de Alginato…
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Bioengineering
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JoVE Journal Bioengineering
Fabricação e Uso de Andaimes Secos de Alginato Macroporoso para Transdução Viral de Células T
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Fabricação e Uso de Andaimes Secos de Alginato Macroporoso para Transdução Viral de Células T

Article DOI: 10.3791/64036-v • 11:16 min • September 9th, 2022
September 9th, 2022 •
1, 1,2, 1,2, 1,2,3
1Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, 2Comparative Medicine Institute, North Carolina State University, 3Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill

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Aqui está um protocolo para a criação de andaimes de alginato macroporoso secos que medeiam a transferência eficiente de genes virais para uso em engenharia genética de células T, incluindo células T para terapia com células CAR-T. Os andaimes demonstraram transduzir células T primárias ativadas com transdução de >85%.

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Bioengenharia Edição 187 andaime de biomateriais alginato macroporoso transdução viral imunoterapia células CAR-T
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