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Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
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Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Article DOI: 10.3791/64070-v • 09:03 min • December 23rd, 2022
December 23rd, 2022 •
*1, *1, 1, 1, 1, 2, 3, 1
1China-USA Citrus Huanglongbing Joint Laboratory, National Navel Orange Engineering Research Center, Gannan Normal University, 2Department of Biological Sciences, Gannan Normal University, 3Citrus Research and Education Center, Department of Microbiology and Cell Science, University of Florida - Institute of Food and Agricultural Sciences
* These authors contributed equally

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Summary
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In this work, a rapid, sensitive, and portable detection method for Candidatus Liberibacter asiaticus based on recombinase polymerase amplification combined with CRISPR-Cas12a was developed.

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Candidatus Liberibacter Asiaticus HLB Citrus Disease CLas Detection Recombinase Polymerase Amplification CRISPR-Cas12a CLas-DETECTR PCR Sensitivity QPCR Comparison Isothermal Condition Handheld Fluorescent Detection Device Solution Preparation Total DNA Isolation Isothermal DNA Amplification Field CLas Detection E.coli Polyethylene Glycol-based Approach
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