3,392 Views
•
08:44 min
•
July 20, 2022
DOI:
Summary
Automatically generated
This protocol is a guide for visualizing dynamic actin and microtubules using an in vitro total internal fluorescence (TIRF) microscopy assay.
Related Videos
12:15
In Vitro Polymerization of F-actin on Early Endosomes
Related Videos
9007 Views
14:14
Visualization of Cortex Organization and Dynamics in Microorganisms, using Total Internal Reflection Fluorescence Microscopy
Related Videos
11508 Views
09:09
Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data
Related Videos
9727 Views
07:20
Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy
Related Videos
2525 Views
06:43
Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins
Related Videos
3414 Views
08:02
Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles
Related Videos
2580 Views
09:45
Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules
Related Videos
10010 Views
07:47
Directly Measuring Forces Within Reconstituted Active Microtubule Bundles
Related Videos
1604 Views
09:10
Reconstituting and Characterizing Actin-Microtubule Composites with Tunable Motor-Driven Dynamics and Mechanics
Related Videos
3248 Views
05:05
TIRF Microscopy to Visualize Actin and Microtubule Coupling Dynamics
Related Videos
219 Views
Read Article
Cite this Article
Henty-Ridilla, J. L. Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence (TIRF) Microscopy. J. Vis. Exp. (185), e64074, doi:10.3791/64074 (2022).
Copy