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JoVE Journal Biochemistry
Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions
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Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

Article DOI: 10.3791/64090-v • 06:32 min • July 28th, 2022
July 28th, 2022 •
*1, *2, 1,3, 1,2,3
1Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, 2Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, 3Department of Biology, University of North Carolina at Chapel Hill
* These authors contributed equally

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Summary
Automatic Translation

Cell-free reconstitution has been a key tool to understand the cytoskeleton assembly, and work in the last decade has established approaches to study septin dynamics in minimal systems. Presented here are three complementary methods to observe septin assembly in different membrane contexts: planar bilayers, spherical supports, and rod supports.

Tags

Membrane Binding Proteins Protein Assemblies Membrane Shape Lipid Bilayer Lipid Diffusion Rates Protein Mobility Liposomes Plasma Cleaning Cover Glass Slides Ceramic Cradle Chamber Preparation PCR Tube UV-activated Adhesive
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