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Biochemistry

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Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions
 

Reconstitution of Septin Assembly at Membranes to Study Biophysical Properties and Functions

Article DOI: 10.3791/64090-v
July 28th, 2022

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Summary July 28th, 2022

Cell-free reconstitution has been a key tool to understand the cytoskeleton assembly, and work in the last decade has established approaches to study septin dynamics in minimal systems. Presented here are three complementary methods to observe septin assembly in different membrane contexts: planar bilayers, spherical supports, and rod supports.

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