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JoVE Journal Biochemistry
La technique du peel-blot
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La technique du peel-blot : une méthode de préparation d’échantillons cryo-EM pour séparer les couches uniques des échantillons biologiques multicouches ou concentrés

Article DOI: 10.3791/64099-v • 07:27 min • June 29th, 2022
June 29th, 2022 •
*1,2, *1, 1,3
1School of Biological Sciences, Georgia Institute of Technology, 2Department of Structural Biology, Genentech, 3School of Chemistry and Biochemistry, Georgia Institute of Technology
* These authors contributed equally

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La technique peel-blot est une méthode de préparation de grille cryo-EM qui permet de séparer des échantillons biologiques multicouches et concentrés en couches uniques afin de réduire l’épaisseur, d’augmenter la concentration des échantillons et de faciliter le traitement des images.

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Biochimie numéro 184
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