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A Simple Benchtop Filtration Method to Isolate Small Extracellular Vesicles from Human Mesenchymal Stem Cells
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A Simple Benchtop Filtration Method to Isolate Small Extracellular Vesicles from Human Mesenchymal Stem Cells

Article DOI: 10.3791/64106-v • 09:10 min • June 23rd, 2022
June 23rd, 2022 •
1,2, 3, 4, 2, 5, 4, 1, 6,7
1Centre for Tissue Engineering and Regenerative Medicine, Universiti Kebangsaan Malaysia Medical Centre, 2My CytoHealth Sdn. Bhd., 3School of Biosciences, Faculty of Health and Medical Sciences, Taylor's University, 4School of Pharmacy, Monash University Malaysia, 5Haematology Unit, Cancer Research Centre, Institute for Medical Research (IMR), National Institutes of Health (NIH), Ministry of Health Malaysia, 6School of Pharmacy, Faculty of Health & Medical Sciences, Taylor's University, 7Centre for Drug Discovery and Molecular Pharmacology (CDDMP), Faculty of Health and Medical Sciences, Taylor's University

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Summary
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This protocol demonstrates how to isolate human umbilical cord-derived mesenchymal stem cells' small extracellular vesicles (hUC-MSC-sEVs) with a simple lab-scale benchtop setting. The size distribution, protein concentration, sEVs markers, and morphology of isolated hUC-MSC-sEVs are characterized by nanoparticle tracking analysis, BCA protein assay, western blot, and transmission electron microscope, respectively.

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Benchtop Filtration Method Small Extracellular Vesicles Human Mesenchymal Stem Cells Protocol Isolate Concentrate Easy Time-effective Benchtop Scale Simple Instruments Large-scale Cell Growth Critical Steps Visual Demonstration Isolation Characterizations Centrifuge Conditioned Medium Cell Debris Removal Supernatant Collection Filtration Centrifugal Filter Unit Phosphate-buffered Saline
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