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Construction of a Human Aorta Smooth Muscle Cell Organ-On-A-Chip Model for Recapitulating Biomechanical Strain in the Aortic Wall
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Bioengineering
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JoVE Journal Bioengineering
Construction of a Human Aorta Smooth Muscle Cell Organ-On-A-Chip Model for Recapitulating Biomechanical Strain in the Aortic Wall

Construction of a Human Aorta Smooth Muscle Cell Organ-On-A-Chip Model for Recapitulating Biomechanical Strain in the Aortic Wall

DOI:
10.3791/64122-v

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11:47 min

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July 06, 2022

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, , , , , , , , , , , ,
1Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital,Fudan University, 2Institutes of Biomedical Sciences and the Shanghai Key Laboratory of Medical Epigenetics, Shanghai Medical College,Fudan University

Chapters

  • 00:04Introduction
  • 01:03Primary Human Aortic Smooth Muscle Cell Isolation
  • 02:53Preparation of PDMS Chip
  • 05:29PDMS Chip Surface Treatment and Sterilization
  • 06:12Cell Seeding on the PDMS Chip and Cell Stretching Process
  • 08:23Preparation of the Mechanical Control System
  • 09:30Results: An HASMC‐Organ‐On‐A‐Chip Model to Precisely Control the Parameters of the Biomechanical Strain on the Aortic Wall
  • 11:03Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we developed a human aorta smooth muscle cell organ-on-a-chip model to replicate the in vivo biomechanical strain of smooth muscle cells in the human aortic wall.

Tags

Keywords: Human AortaSmooth Muscle CellsOrgan-on-a-chipBiomechanical StrainAortic WallIn Vitro ModelDrug ScreeningPersonalized MedicineAortic DiseasesCell CulturePDMSMold Design

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