In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Marcos Ezequiel Gramajo; Ryan J. Lake; Yi Lu; Ana Sol Peinetti

doi:10.3791/64127

Journal

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Bioengineering

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In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

JoVE Journal
Bioengineering
Author Produced

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JoVE Journal Bioengineering

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

1,645 Views

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12:23 min

•

September 07, 2022

DOI:

10.3791/64127-v

DOI:

10.3791/64127-v

•

12:23 min

September 07, 2022

•

1631 Views

Marcos Ezequiel Gramajo*¹, Ryan J. Lake*², Yi Lu³, Ana Sol Peinetti¹

¹INQUIMAE (CONICET), Departamento de Química Inorgánica, Analítica y Química Física, Facultad de Ciencias Exactas y Naturales,Universidad de Buenos Aires ²Department of Chemistry,University of Illinois at Urbana-Champaign ³Department of Chemistry,University of Texas at Austin

Summary

We provide a protocol that can be generally applied to select aptamers that bind to infectious viruses only and not to viruses that have been rendered non-infectious by a disinfection method or to any other similar viruses. This opens the possibility of determining infectivity status in portable and rapid tests.