Journal
/
Biology
/
Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells
JoVE Journal
Biology
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells

Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells

DOI:
10.3791/64135-v

•

07:03 min

•

September 23, 2022

•

, , , , , , ,
1College of Life Science,Northwest University, 2State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology,Fourth Military Medical University, 3Xi’an Institute of Tissue Engineering and Regenerative Medicine, 4School of Basic Medicine,Fourth Military Medical University, 5Department of Orthodontics, School of Stomatology,Fourth Military Medical University

Chapters

  • 00:05Introduction
  • 01:04Differential Centrifugation for Isolation of Exosomes and Microvesicles
  • 02:25Detection of Particle Numbers and Size Distribution of Extracellular Vesicles
  • 03:29Labeling of Extracellular Vesicles Derived from Mesenchymal Stem Cells
  • 04:50Systemic Injection of Extracellular Vesicles Derived from Mesenchymal Stem Cells
  • 05:24Results: Characterization of Extracellular Vesicles Derived from Mesenchymal Stem Cells
  • 06:19Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol describes the differential centrifugation for isolating and characterizing representative EVs (exosomes and microvesicles) from cultured human MSCs. Further applications of these EVs are also explained in this article.

Tags

Keywords: Extracellular VesiclesMesenchymal Stem CellsIsolationCharacterizationDifferential CentrifugationCell CultureTherapeutic ApplicationPKH26 DyeParticle Tracking Analysis

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/64135?article_type=v

Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells (MSCs) and Endothelial Colony Forming Progenitor Cells (ECFCs)
check_url/64135?article_type=v

Isolation of Stem Cells from Human Pancreatic Cancer Xenografts
check_url/64135?article_type=v

Isolation and Culture of Adult Epithelial Stem Cells from Human Skin
check_url/64135?article_type=v

Isolation & Characterization of Hoechstlow CD45negative Mouse Lung Mesenchymal Stem Cells
check_url/64135?article_type=v

Naïve Adult Stem Cells Isolation from Primary Human Fibroblast Cultures
check_url/64135?article_type=v

Induction and Analysis of Epithelial to Mesenchymal Transition
check_url/64135?article_type=v

Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates
check_url/64135?article_type=v

Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor
check_url/64135?article_type=v

Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells
check_url/64135?article_type=v

Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria

Read Article