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JoVE Journal Neuroscience
Isolatie en directe neuronale herprogrammering van muisastryoten
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Isolatie en directe neuronale herprogrammering van muisastryoten

Article DOI: 10.3791/64175-v • 07:25 min • July 7th, 2022
July 7th, 2022 •
1,2,3, 2, 1,2
1Institute of Stem Cell Research, Helmholtz Zentrum München, German Research Center for Environmental Health, 2Department of Physiological Genomics, Biomedical Center Munich, Ludwig-Maximilians University, 3Graduate School of Systemic Neurosciences, BioCenter, Ludwig-Maximilians University

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Hier beschrijven we een gedetailleerd protocol om sterk verrijkte culturen van astrocyten te genereren die zijn afgeleid van verschillende regio's van het centrale zenuwstelsel van postnatale muizen en hun directe omzetting in functionele neuronen door de geforceerde expressie van transcriptiefactoren.

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Neurowetenschappen Nummer 185
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