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JoVE Journal Neuroscience
Isolement et reprogrammation neuronale directe des astrocytes de souris
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Isolement et reprogrammation neuronale directe des astrocytes de souris

Article DOI: 10.3791/64175-v • 07:25 min • July 7th, 2022
July 7th, 2022 •
1,2,3, 2, 1,2
1Institute of Stem Cell Research, Helmholtz Zentrum München, German Research Center for Environmental Health, 2Department of Physiological Genomics, Biomedical Center Munich, Ludwig-Maximilians University, 3Graduate School of Systemic Neurosciences, BioCenter, Ludwig-Maximilians University

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Nous décrivons ici un protocole détaillé pour générer des cultures hautement enrichies d’astrocytes dérivés de différentes régions du système nerveux central de souris postnatales et leur conversion directe en neurones fonctionnels par l’expression forcée de facteurs de transcription.

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Neurosciences numéro 185
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