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JoVE Journal Neuroscience
Isolamento e riprogrammazione neuronale diretta degli astrociti di topo
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Isolamento e riprogrammazione neuronale diretta degli astrociti di topo

Article DOI: 10.3791/64175-v • 07:25 min • July 7th, 2022
July 7th, 2022 •
1,2,3, 2, 1,2
1Institute of Stem Cell Research, Helmholtz Zentrum München, German Research Center for Environmental Health, 2Department of Physiological Genomics, Biomedical Center Munich, Ludwig-Maximilians University, 3Graduate School of Systemic Neurosciences, BioCenter, Ludwig-Maximilians University

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Qui descriviamo un protocollo dettagliato per generare colture altamente arricchite di astrociti derivati da diverse regioni del sistema nervoso centrale dei topi postnatali e la loro conversione diretta in neuroni funzionali mediante l'espressione forzata di fattori di trascrizione.

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Neuroscienze Numero 185
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