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Isolamento e Reprogramação Neuronal Direta de Astrócitos de Camundongos
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Isolamento e Reprogramação Neuronal Direta de Astrócitos de Camundongos

Article DOI: 10.3791/64175-v • 07:25 min • July 7th, 2022
July 7th, 2022 •
1,2,3, 2, 1,2
1Institute of Stem Cell Research, Helmholtz Zentrum München, German Research Center for Environmental Health, 2Department of Physiological Genomics, Biomedical Center Munich, Ludwig-Maximilians University, 3Graduate School of Systemic Neurosciences, BioCenter, Ludwig-Maximilians University

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Aqui descrevemos um protocolo detalhado para gerar culturas altamente enriquecidas de astrócitos derivados de diferentes regiões do sistema nervoso central de camundongos pós-natais e sua conversão direta em neurônios funcionais pela expressão forçada de fatores de transcrição.

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Neurociência Edição 185
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