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1Key Laboratory of Sustainable Forest Ecosystem Management – Ministry of Education,Northeast Forestry University, 2Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences
Chapters
- 00:05Introduction
- 00:33Target Design and In Vitro Synthesis of sgRNA
- 01:18Embryo Collection and Preparation
- 02:39Microinjection of Embryos
- 04:24Post-Injection Insect Rearing
- 05:07Mutant Screening
- 06:41Results: Mutant Screening in B. dorsalis
- 07:39Conclusion
This paper presents the step-by-step protocols for CRISPR/Cas9 mutagenesis of the Oriental fruit fly Bactrocera dorsalis. Detailed steps provided by this standardized protocol will serve as a useful guide for generating mutant flies for functional gene studies in B. dorsalis.
