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JoVE Journal Neuroscience
Despolarização Seletiva Assistida por Microfluídica das Mitocôndrias Axonais
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Despolarização Seletiva Assistida por Microfluídica das Mitocôndrias Axonais

Article DOI: 10.3791/64196-v • 06:55 min • August 4th, 2022
August 4th, 2022 •
*1,2, *1,2, 2,3,4
1TUM Medical Graduate Center, Technical University of Munich, 2Max Planck Institute of Neurobiology, 3TUM School of Medicine, Institute of Neuronal Cell Biology, Technical University of Munich, 4Munich Cluster for Systems Neurology
* These authors contributed equally

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O presente protocolo descreve a semeadura e coloração de mitocôndrias neuronais em câmaras microfluídicas. O gradiente de pressão fluídica nessas câmaras permite o tratamento seletivo de mitocôndrias em axônios para analisar suas propriedades em resposta a desafios farmacológicos sem afetar o compartimento do corpo celular.

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Neurociência Edição 186 Axônio mitocôndrias microfluídica neurodegeneração
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