Journal
/
Biochemistry
/
Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

DOI:
10.3791/64200-v

•

09:49 min

•

November 18, 2022

•

, , , ,
1Department of Chemistry,University of Florida, 2Division of Protective Immunity,Children’s Hospital of Philadelphia, 3Department of Pathology and Laboratory Medicine, Perelman School of Medicine,University of Pennsylvania

Chapters

  • 00:05Introduction
  • 00:58Ambient, Vacuum, and Heat-Mediated Drying of Clostridioides difficile and Enterococcus faecalis Bacterial Macrocolonies
  • 02:33MALDI Matrix Application via Robotic Spraying
  • 03:12Preparing Bacterial Macrocolonies for MALDI Imaging Mass Spectrometry Data Acquisition
  • 04:37Imaging Mass Spectrometry Data Analysis and Compound Identification
  • 05:57Cryosectioning of Noninfected Control Versus Clostridioides difficile-Infected Mouse Cecal Tissues
  • 07:45Preparation of Noninfected Control Versus Clostridioides difficile-Infected Mouse Cecal Tissues for Matrix Application and MALDI Imaging Mass Spectrometry
  • 08:08Results: Imaging Mass Spectrometry Analysis of Bacterial Macrocolonies and Infected Murine Cecal Tissues
  • 09:09Conclusion

Summary

Automatic Translation

Automatic Translation

A novel sample preparation method is demonstrated for the analysis of agar-based, bacterial macrocolonies via matrix-assisted laser desorption/ionization imaging mass spectrometry.

Tags

Keywords: Microbial CooperationImaging Mass SpectrometryBacterial ColoniesAgarMALDI MatrixSample PreparationVacuum DryingSpatial MetabolismMetabolitesMicrobial Pathogens

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/64200?article_type=v

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
check_url/64200?article_type=v

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
check_url/64200?article_type=v

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
check_url/64200?article_type=v

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
check_url/64200?article_type=v

Assessing the Viability of a Synthetic Bacterial Consortium on the In Vitro Gut Host-microbe Interface
check_url/64200?article_type=v

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
check_url/64200?article_type=v

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues
check_url/64200?article_type=v

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
check_url/64200?article_type=v

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain
check_url/64200?article_type=v

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers

Read Article