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In Vivo Luminal Measurement of Distension-Evoked Urothelial ATP Release in Rodents

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Medicine

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JoVE Journal Medicine

In Vivo Luminal Measurement of Distension-Evoked Urothelial ATP Release in Rodents

In Vivo Luminal Measurement of Distension-Evoked Urothelial ATP Release in Rodents

DOI:

10.3791/64227-v

•

09:17 min

•

September 07, 2022

•

Stephanie L. Daugherty, Keara M. Healy, Jonathan M. Beckel

¹Department of Pharmacology and Chemical Biology,University of Pittsburgh School of Medicine

Chapters

00:05Introduction
00:35Anesthesia and Ganglionic Block
01:29Surgical Procedure: Suprapubic Bladder Catheterization
02:29Transurethral Catheterization
03:43Experimental Setup
05:08Collection of Samples
06:20Quantifying ATP from Collected Samples
07:03Results: Measurement of Urothelial ATP Release In Vivo from the Lumen of the Bladder
08:39Conclusion

Summary

Automatic Translation

This protocol describes the procedure for measuring ATP concentrations in the lumen of the bladder in an anesthetized rodent.

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