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Journal / Genetics / 出芽酵母における近接ライゲーションと定量PCRによる相同組換え中間体の検出…
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JoVE Journal Genetics
出芽酵母における近接ライゲーションと定量PCRによる相同組換え中間体の検出
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出芽酵母における近接ライゲーションと定量PCRによる相同組換え中間体の検出

Article DOI: 10.3791/64240-v • 07:55 min • September 11th, 2022
September 11th, 2022 •
1, 2, 2, 1,3
1Department of Microbiology & Molecular Genetics, University of California, Davis, 2Laboratory of Biology & Modeling of the Cell, École Normale Supérieure de Lyon, 3Department of Molecular & Cellular Biology, University of California, Davis

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D-ループキャプチャー(DLC)およびD-ループ伸長(DLE)アッセイは、近接ライゲーションの原理と定量的PCRを利用して、 出芽酵母の誘導性二本鎖切断部位でのDループ形成、Dループ伸長、および産物形成を定量化します。

Tags

遺伝学、第187号、Dループ、ゲノム安定性、関節分子、Rad51、ブレーク誘導複製
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