Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Single-Molecule Diffusion and Assembly on…
JoVE Journal
Biochemistry
Author Produced

A subscription to JoVE is required to view this content.

JoVE Journal Biochemistry
Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes
Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei
Journal (Biochemistry)
Isolation of F1-ATPase from the Parasitic Protist Trypanosoma brucei
Protein Complex Affinity Capture from Cryomilled Mammalian Cells
Journal (Biochemistry)
Protein Complex Affinity Capture from Cryomilled Mammalian Cells
 

Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes

Article DOI: 10.3791/64243-v • 10:43 min • July 19th, 2022
July 19th, 2022 •
1, 1, 1, 1,2, 1, 1,3
1Department of Chemical Engineering, Indian Institute of Science, 2Holonyak Micro & Nanotechnology Lab, University of Illinois, Urbana-Champaign, 3Center for BioSystems Science and Engineering, Indian Institute of Science

Chapters

Summary
Automatic Translation

Here, a protocol to perform and analyze the binding, mobility, and assembly of single molecules on artificial crowded lipid membranes using single-molecule total internal reflection fluorescence (smTIRF) microscopy is presented.

Tags

Single-molecule Diffusion Assembly Polymer-crowded Lipid Membranes Synthetic Crowded Supported Lipid Bilayers Membrane Biomolecular Reactions Binding Diffusion Assembly Substrate Cleaning Bilayer Damage Avoidance Signal To Noise Ratio Single Molecule Imaging Acrylic Slide Laser Cut Tape Plasma Treatment Microfluidic Imaging Chambers Desiccated Conditions Chloroform Solution POPC DOPE-PEG(2000) Mole Fraction
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter