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Chapters
- 00:04Introduction
- 00:43Sample Extraction
- 01:47Sample Processing for Analysis of Hematopoietic Cell Populations in Total Bone Marrow
- 03:19Sample Processing for the Analysis of Stromal Cell Populations in Total Bone Marrow
- 05:13Sample Processing for the Analysis of Hematopoietic Cell Populations in Crushed and Flushed Bone Marrow
- 06:28Results: Phenotypic Analysis of Murine Bone Marrow Hematopoietic Stem and Progenitor Cells and Stromal Niche Cells by Flow Cytometry
- 07:40Conclusion
Here, we describe a simple protocol for the isolation and staining of murine bone marrow cells to phenotype hemopoietic stem and progenitor cells along with the supporting niche endothelial and mesenchymal stem cells. A method to enrich cells located in endosteal and central bone marrow areas is also included.
