Journal
/
/
Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ
Automated Analysis of Intracellular Phenotypes of <em>Salmonella</em> Using ImageJ
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ

Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ

2,813 Views

10:39 min

August 09, 2022

DOI:

10:39 min
August 09, 2022

2802 Views
, ,

Summary

Automatically generated

Salmonella invades and replicates inside intestinal epithelial cells both in Salmonella-specific vacuoles and free in the cytosol (hyper-replication). A high-throughput fluorescence microscopy-based protocol is described here to quantify the intracellular phenotypes of Salmonella by two complementary image analyses through ImageJ, reaching single-cell resolution and scoring.

Related Videos

Read Article