Automated Analysis of Intracellular Phenotypes of <em>Salmonella</em> Using ImageJ

Melissa Berni; Stefano Pongolini; Martina Tambassi

doi:10.3791/64263

Journal

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Immunology and Infection

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Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ

Automated Analysis of Intracellular Phenotypes of <em>Salmonella</em> Using ImageJ

JoVE Journal
Immunology and Infection

This content is Free Access.

JoVE Journal Immunology and Infection

Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ

Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ

2,813 Views

•

10:39 min

•

August 09, 2022

DOI:

10.3791/64263-v

DOI:

10.3791/64263-v

•

10:39 min

August 09, 2022

•

2802 Views

Melissa Berni¹, Stefano Pongolini¹, Martina Tambassi¹

¹Risk Analysis and Genomic Epidemiology Unit,Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia-Romagna (IZSLER)

Summary

Salmonella invades and replicates inside intestinal epithelial cells both in Salmonella-specific vacuoles and free in the cytosol (hyper-replication). A high-throughput fluorescence microscopy-based protocol is described here to quantify the intracellular phenotypes of Salmonella by two complementary image analyses through ImageJ, reaching single-cell resolution and scoring.