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Immunology and Infection
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Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ

Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ

DOI:
10.3791/64263-v

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10:39 min

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August 09, 2022

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, ,
1Risk Analysis and Genomic Epidemiology Unit,Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia-Romagna (IZSLER)

Chapters

  • 00:04Introduction
  • 00:53Single-Cell Analysis
  • 08:33Results: Single-Cell and Area Analysis of Salmonella
  • 10:09Conclusion

Summary

Automatic Translation

Automatic Translation

Salmonella invades and replicates inside intestinal epithelial cells both in Salmonella-specific vacuoles and free in the cytosol (hyper-replication). A high-throughput fluorescence microscopy-based protocol is described here to quantify the intracellular phenotypes of Salmonella by two complementary image analyses through ImageJ, reaching single-cell resolution and scoring.

Tags

Keywords: Fluorescence MicroscopySalmonellaIntracellular PhenotypesImageJAutomated AnalysisHigh-throughputSingle-cell AnalysisCell SegmentationContrast EnhancementMaxima DetectionCell MaskingThresholding

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