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Immunology and Infection
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Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

Article DOI: 10.3791/64279-v • 09:14 min • September 28th, 2022
September 28th, 2022 •
1, 1, 1,2
1Department of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine, American University of Beirut, 2Center for Infectious Diseases Research (CIDR), Faculty of Medicine, American University of Beirut

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Summary
Automatic Translation

This protocol permits the isolation of Epstein-Barr virus particles from the human P3HR1 cell line upon inducing the viral lytic cycle with phorbol 12-myristate 13-acetate. DNA is subsequently extracted from the viral preparation and subjected to real-time PCR to quantify the viral particle concentration.

Tags

Isolation Quantification Epstein-Barr Virus P3HR1 Cell Line Virus Stock Research Purposes Technique Easy Inexpensive Fast Reliable Method EB Viral Stock In Vitro In Vivo Experimental Models Diagnosis Of EB Cell Suspension A Culture Medium Dimethyl Sulfoxide (DMSO) PMA (phorbol 12-myristate 13-acetate) Concentration Total Volume Culture Plate Cell Culture Incubator Centrifuge Cell-free Virus Containing Supernatant
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