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Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

DOI:
10.3791/64279-v

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09:14 min

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September 28, 2022

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, ,
1Department of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine,American University of Beirut, 2Center for Infectious Diseases Research (CIDR), Faculty of Medicine,American University of Beirut

Chapters

  • 00:05Introduction
  • 00:36Preparing the Plate for Culture
  • 01:49Induction and Isolation of Epstein-Barr Virus Particles
  • 02:45DNA Extraction from Viral Particles
  • 04:45Checking DNA Concentration and Purity
  • 05:34Quantification by Real-Time Polymerase Chain Reaction
  • 07:54Results: Cell Counting and Induction of P3HR-1 Cells with PMA and DMSO
  • 08:48Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol permits the isolation of Epstein-Barr virus particles from the human P3HR1 cell line upon inducing the viral lytic cycle with phorbol 12-myristate 13-acetate. DNA is subsequently extracted from the viral preparation and subjected to real-time PCR to quantify the viral particle concentration.

Tags

Keywords: Epstein-Barr VirusP3HR1 Cell LineViral StockVirus IsolationVirus QuantificationPMADMSOCell CultureCentrifugationPhenol-chloroform ExtractionEthanol Precipitation

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