A Modified Technique for Arteriovenous Fistula Construction in Rabbits

Liu Zhen; Hou Guocun; Wang Xiaohe; Hu Jingfang; Li Jie; Wei Minggang

doi:10.3791/64352

A Modified Technique for Arteriovenous Fistula Construction in Rabbits

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JoVE Journal Medicine

A Modified Technique for Arteriovenous Fistula Construction in Rabbits

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05:00 min

February 10, 2023

DOI: 10.3791/64352-v

Liu Zhen*^1,2,3, Hou Guocun*³, Wang Xiaohe³, Hu Jingfang³, Li Jie³, Wei Minggang^1,2

¹Department of Traditional Chinese Medicine,The First Affiliated Hospital of Soochow University, ²Department of Nephrology,The First Affiliated Hospital of Soochow University, ³Department of Nephrology,Suzhou Science & Technology Town Hospital

The present protocol proposes the creation of an arteriovenous fistula in rabbits using a modified no-touch technique. The technique involves the side-to-side anastomosis of the common carotid artery and external jugular vein without the dissection of the perivenous tissues or cutting off the artery.

Clinically, radiocephalic AVF created with a modified no-touch technique can increase primary patency and decrease juxta-anastomotic stenosis. To explore the mechanism of MNTT, this animal model needs to be established. The main advantage of the modified no-touch technique is to reduce injury to the external jugular vein when establishing AVF.

When dealing with the external jugular vein care should be taken to preserve the paravenous tissue as much as possible and reduce the injury to the vein. To begin, place an anesthetized rabbit in a supine position on a fixed table. Bind its limbs and incisors.

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