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JoVE Journal
Developmental Biology

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Identification and Isolation of Burst-Forming Unit and Colony-Forming Unit Erythroid Progenitors from Mouse Tissue by Flow Cytometry
 

Identification and Isolation of Burst-Forming Unit and Colony-Forming Unit Erythroid Progenitors from Mouse Tissue by Flow Cytometry

Article DOI: 10.3791/64373-v 08:31 min November 4th, 2022
November 4th, 2022

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Summary

Here, we describe a novel flow cytometric method for prospective isolation of early burst-forming unit erythroid (BFU-e) and colony-forming unit erythroid (CFU-e) progenitors directly from fresh mouse bone marrow and spleen. This protocol, developed based on single-cell transcriptomic data, is the first to isolate all the tissue's erythroid progenitors with high purity.

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Keywords: Burst-Forming Unit (BFU-E) Colony-Forming Unit (CFU-E) Erythroid Progenitors Flow Cytometry Bone Marrow Spleen Tissue Isolation Cell Staining Fluorescence Minus One (FMO) Controls Single-Color Controls
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