Journal
/
Bioengineering
/
In Vitro Cleavage Assays using Purified Recombinant Drosophila Caspases for Substrate Screening
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
In Vitro Cleavage Assays using Purified Recombinant Drosophila Caspases for Substrate Screening

In Vitro Cleavage Assays using Purified Recombinant Drosophila Caspases for Substrate Screening

DOI:
10.3791/64392-v

•

08:16 min

•

October 06, 2022

•

,
1Department of Molecular, Cell and Cancer Biology,UMass Chan Medical School

Chapters

  • 00:04Introduction
  • 01:15Small‐Scale Recombinant Caspases Extraction
  • 02:34Small‐Scale His‐Tagged Caspases Purification
  • 04:09In Vitro Cleavage Assay with Substrates Generated with RRL
  • 05:29Results: In Vitro Cleavage Assays to Express and Purify Recombinant Drosophila Caspases
  • 07:33Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a protocol to express and purify recombinant Drosophila caspases Dronc and Drice, and their use in in vitro cleavage assays.

Tags

In Vitro Cleavage AssayCaspasesDrosophilaDroncRecombinant ProteinsSubstrate ScreeningCell LysisNi-NTA AgarosePurificationEnzymatic Activity

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

Flexural Rigidity Measurements of Biopolymers Using Gliding Assays

Using Microfluidics Chips for Live Imaging and Study of Injury Responses in Drosophila Larvae

Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification

Eye Irritation Test (EIT) for Hazard Identification of Eye Irritating Chemicals using Reconstructed Human Cornea-like Epithelial (RhCE) Tissue Model

An In Vitro Organ Culture Model of the Murine Intervertebral Disc

Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate (LAL) Assays

Applications for Open Source Microplate-Compatible Illumination Panels

In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding

Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure

Read Article