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In Vitro Cleavage Assays using Purified Recombinant Drosophila Caspases for Substrate Screening
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
In Vitro Cleavage Assays using Purified Recombinant Drosophila Caspases for Substrate Screening
DOI:

08:16 min

October 06, 2022

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Chapters

  • 00:04Introduction
  • 01:15Small‐Scale Recombinant Caspases Extraction
  • 02:34Small‐Scale His‐Tagged Caspases Purification
  • 04:09In Vitro Cleavage Assay with Substrates Generated with RRL
  • 05:29Results: In Vitro Cleavage Assays to Express and Purify Recombinant Drosophila Caspases
  • 07:33Conclusion

Summary

Automatic Translation

Here we present a protocol to express and purify recombinant Drosophila caspases Dronc and Drice, and their use in in vitro cleavage assays.

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