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A High-Throughput Platform for Culture and 3D Imaging of Organoids
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JoVE Journal Biology
A High-Throughput Platform for Culture and 3D Imaging of Organoids

A High-Throughput Platform for Culture and 3D Imaging of Organoids

DOI:
10.3791/64405-v

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07:42 min

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October 14, 2022

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, , , , , , ,
1Mechanobiology Institute (MBI),National University of Singapore, 2Biomedical Engineering Department,National University of Singapore, 3Department of Biological Sciences,National University of Singapore, 4IRL 3639 CNRS, 5Department of Microbiology & Immunology, Immunology Translational Research Program, Yong Loo Lin School of Medicine,National University of Singapore

Chapters

  • 00:04Introduction
  • 00:49PDMS Mold Dicing and Production of the UV‐Curable, Adhesive, Textured Layer
  • 02:19Coverslip Substrate Preparation
  • 03:34Transferring the Textured Film to the Final Substrate
  • 04:33Cell Seeding, Release, and Collection of the Organoids
  • 06:29Results: Fabrication of the Microwell Culture Dish for a High‐Density Organoid Culture and Differentiation
  • 07:00Conclusion

Summary

Automatic Translation

Automatic Translation

This paper presents a fabrication protocol for a new type of culture substrate with hundreds of microcontainers per mm2, in which organoids can be cultured and observed using high-resolution microscopy. The cell seeding and immunostaining protocols are also detailed.

Tags

High-throughputOrganoid Culture3D ImagingMicrotexture Cell Culture DishPDMS MoldUV Curable AdhesiveSpin CoatingPlasma TreatmentBiomedical ApplicationsDrug ScreeningCancer Research

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