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Phage-Mediated Genetic Manipulation of the Lyme Disease Spirochete Borrelia burgdorferi
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Chapters
- 00:04Introduction
- 00:40Preparation of Borrelia burgdorferi Culture for the Production of φBB-1 and Induction of Borrelia burgdorferi Phage φBB-1
- 02:31Polyethylene Glycol Precipitation to Recover Phage for Use in Transduction Assay
- 05:34Transduction Assay Following Polyethylene Glycol Precipitation of φBB-1 and Selection of Transductants
- 06:51Results: Transducing Heterologous DNA Between Different Strains of the Lyme Disease Spirochete Using Borrelia burgdorferi Bacteriophage
- 08:12Conclusion
The ability of bacteriophage to move DNA between bacterial cells makes them effective tools for the genetic manipulation of their bacterial hosts. Presented here is a methodology for inducing, recovering, and using φBB-1, a bacteriophage of Borrelia burgdorferi, to transduce heterologous DNA between different strains of the Lyme disease spirochete.
