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Fagmedieret genmanipulation af borreliose Spirochete Borrelia burgdorferi
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Chapters
- 00:04Introduction
- 00:40Preparation of Borrelia burgdorferi Culture for the Production of φBB-1 and Induction of Borrelia burgdorferi Phage φBB-1
- 02:31Polyethylene Glycol Precipitation to Recover Phage for Use in Transduction Assay
- 05:34Transduction Assay Following Polyethylene Glycol Precipitation of φBB-1 and Selection of Transductants
- 06:51Results: Transducing Heterologous DNA Between Different Strains of the Lyme Disease Spirochete Using Borrelia burgdorferi Bacteriophage
- 08:12Conclusion
Bakteriofagens evne til at flytte DNA mellem bakterieceller gør dem til effektive værktøjer til genetisk manipulation af deres bakterielle værter. Præsenteret her er en metode til at inducere, genoprette og bruge φBB-1, en bakteriofag af Borrelia burgdorferi, til at transducere heterologt DNA mellem forskellige stammer af Lyme-sygdommen spirochete.
