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Chapters
- 00:04Introduction
- 00:51Inoculation, Culturing, and Subculturing of C. albicans
- 01:29Preparation of the Peptide Solutions in a 96-Well Plate
- 02:33Dilution of the C. albicans Subculture
- 03:37Incubation of C. albicans</i with Peptide Solutions and Preparation of the Cells for the Quantification of Viability
- 05:00Determination of Antifungal Activity
- 05:34Results: Assessment of the Antifungal Activity of Histatin 5 and Variants of Histatin 5 Containing Modifications at the Lysine Residues
- 06:05Conclusion
This protocol describes a method for obtaining quantitative data on the antifungal activity of peptides and other compounds, such as small-molecule antifungal agents, against Candida albicans. Its use of optical density rather than counting colony-forming units to quantify growth inhibition saves time and resources.
