Extrahepatic Bile Duct and Gall Bladder Dissection in Nine-Day-Old Mouse Neonates

Hans  Christian Schmidt; Johanna Hagens; Pauline Schuppert; Clara Philippi; Konrad Reinshagen; Christian Tomuschat

doi:10.3791/64424

Extrahepatic Bile Duct and Gall Bladder Dissection in Nine-Day-Old Mouse Neonates

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JoVE Journal Medicine

Extrahepatic Bile Duct and Gall Bladder Dissection in Nine-Day-Old Mouse Neonates

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06:10 min

August 23, 2022

DOI: 10.3791/64424-v

Hans Christian Schmidt^1,2,3, Johanna Hagens^1,2,3, Pauline Schuppert^1,2, Clara Philippi^1,2, Konrad Reinshagen^1,2, Christian Tomuschat^1,2

¹Department of Pediatric Surgery,University Medical Center Hamburg-Eppendorf, ²Research Laboratory for Pediatric Surgery, ³Research Animal Facility,University Medical Center Hamburg-Eppendorf

Overview

This article presents a novel technique for isolating the entire extrahepatic bile duct system in murine neonates, crucial for studying bile duct disorders. The method ensures the integrity of the bile duct during dissection, addressing previous issues of comparability in sample collection.

Key Study Components

Area of Science

Neuroscience
Biology
Developmental Biology

Background

Previous reports lacked clarity on sample collection methods.
Contaminating cells can be removed atraumatically with the new technique.
The protocol is applicable to various experimental approaches.
Maintaining stomach attachment aids in research orientation.

Purpose of Study

To develop a reliable dissection protocol for the extrahepatic biliary system.
To enhance comparability in research on murine neonatal bile duct disorders.
To provide a step-by-step approach for effective training.

Methods Used

Dissection of the entire extrahepatic biliary system.
Atraumatic removal of contaminating cells.
Step-by-step training protocol for researchers.
Orientation techniques using stomach and duodenum.

Main Results

The technique allows for complete dissection of the biliary system.
Improved sample integrity and comparability across studies.
Facilitates research on related disorders like duodenal atresia.
Training enhances speed and outcomes in dissections.

Conclusions

The new dissection protocol is effective for murine neonatal studies.
It addresses previous limitations in sample collection methods.
Future research can benefit from this standardized approach.

Frequently Asked Questions

What is the main advantage of the new dissection technique?

The main advantage is the ability to dissect the entire extrahepatic biliary system while removing contaminating cells atraumatically.

How does this technique improve comparability in research?

By providing a standardized method for sample collection, it reduces variability and enhances the reliability of results across studies.

What training is required for this dissection protocol?

Initial training may take time, but following the step-by-step approach will improve speed and outcomes.

Can this technique be applied to other research areas?

Yes, it can be adapted for studies related to duodenal atresia and other biliary disorders.

What are the key components of the dissection protocol?

Key components include maintaining stomach attachment for orientation and ensuring atraumatic removal of cells.

Is this technique suitable for all researchers?

Yes, with proper training, researchers at various levels can effectively use this technique.

For the observation of murine neonatal bile duct disorders, an intact bile duct and efficient preparation are required. Therefore, a new approach for isolating the entire extrahepatic bile duct system in murine neonates was successfully developed while maintaining the integrity of the bile duct.

Many reports assessing the extrahepatic biliary system reported that they obtained the samples but not how they obtained them, raising issues regarding comparability. The main advantage of this technique is that the entire extrahepatic biliary system can be dissected and contaminating cells can be removed atraumatically. Our dissection protocol can be generally applied to experimental approaches working on murine neonatal bile duct disorders, aiming for the dissection of the extrahepatic biliary system.

Some features of the protocol, for example, leaving the stomach attached to the duodenum, might be useful in research of duodenal atresia to allow correct orientation by the partially-digested milk expressed by the stomach. At the beginning, the technique may necessitate some time for training. Practicing and following the step-by-step approach will increase speed and outcome.

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