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Cultivation Methods of Spirochetes from Borrelia burgdorferi Sensu Lato Complex and Relapsing Fever Borrelia
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Immunology and Infection
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JoVE Journal Immunology and Infection
Cultivation Methods of Spirochetes from Borrelia burgdorferi Sensu Lato Complex and Relapsing Fever Borrelia

Cultivation Methods of Spirochetes from Borrelia burgdorferi Sensu Lato Complex and Relapsing Fever Borrelia

DOI:
10.3791/64431-v

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10:37 min

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November 25, 2022

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, , , , , ,
1Department of Biology,Mt. Allison University, 2Department of Molecular Biology,Umeå University, 3Biology Centre Czech Academy of Sciences,Institute of Parasitology

Chapters

  • 00:04Introduction
  • 01:39Preparation of Complete Barbour-Stoenner-Kelly (BSK)-II Medium
  • 02:48Culturing Borrelia with or Without Antibiotics and Long-Term Storage
  • 05:48Isolation of Spirochetes from Environmental or Clinical Sources
  • 07:30Isolation of Monoclonal Populations of B. burgdorferi Sensu Lato Spirochetes and B. miyamotoi from Liquid Cultures by Cultivation on Solid Media
  • 08:39Results: Culturing of Diverse Borrelia Spirochetes
  • 09:23Conclusion

Summary

Automatic Translation

Automatic Translation

In vitro culture is a direct detection method for the presence of living bacteria. This protocol describes methods for the culture of diverse Borrelia spirochetes, including those of the Borrelia burgdorferi sensu lato complex, relapsing fever Borrelia species, and Borrelia miyamotoi. These species are fastidious and slow growing but can be cultured.

Tags

Keywords: SpirochetesBorrelia BurgdorferiRelapsing Fever BorreliaBorrelia MiyamotoiCultureBSK-II MediumBacterial GrowthRabbit SerumGelatinAnaerobic AtmosphereIncubation Temperature

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