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Developmental Biology

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JoVE Journal Developmental Biology
Ablation laser multiphotonique de centres organisateurs de microtubules cytoplasmiques dans les ovocytes de souris
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Ablation laser multiphotonique de centres organisateurs de microtubules cytoplasmiques dans les ovocytes de souris

Article DOI: 10.3791/64439-v • 08:24 min • November 11th, 2022
November 11th, 2022 •
1,2, 3, 1
1Animal Sciences Research Center, University of Missouri, 2Universidad Nacional de Colombia, 3Advanced Light Microscopy Core, University of Missouri

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Un protocole optimisé est présenté qui permet l’épuisement des centres d’organisation des microtubules cytoplasmiques dans les ovocytes de souris au cours de la métaphase I à l’aide d’un laser femtoseconde proche infrarouge.

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Biologie du développement numéro 189 ovocyte MTOC cytoplasmique en métaphase méiose ablation au laser
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