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Establishing 3D Endometrial Organoids from the Mouse Uterus
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Establishing 3D Endometrial Organoids from the Mouse Uterus

Establishing 3D Endometrial Organoids from the Mouse Uterus

DOI:
10.3791/64448-v

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06:24 min

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January 06, 2023

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, , , , ,
1Department of Pathology & Immunology,Baylor College of Medicine, 2Center for Drug Discovery,Baylor College of Medicine, 3Departments of Molecular Virology and Microbiology and Medicine,Baylor College of Medicine

Chapters

  • 00:05Introduction
  • 00:40Isolation of Uterine Epithelium from Mice Using Enzymatic and Mechanical Methods
  • 03:18Encapsulation of Uterine Epithelium into Gel Matrix to Establish Organoids
  • 04:19Results: Gene Expression and Histological Analyses of Mouse Endometrial Epithelial Organoids
  • 05:32Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes methodologies to establish mouse endometrial epithelial organoids for gene expression and histological analyses.

Tags

Keywords: 3D Endometrial OrganoidsMouse UterusUterine EpitheliumEnzymatic DissociationMechanical DissociationCell CultureOrganoid EstablishmentCell PelletCollagenaseDNA Solution

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