Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment

Adeel Ahmed; Indranil M. Joshi; Madeleine R. Goulet; Justin A. Vidas; Ann M. Byerley; Mehran Mansouri; Steven W. Day; Vinay V. Abhyankar

doi:10.3791/64457

Journal

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Bioengineering

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Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment

2,193 Views

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07:12 min

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September 07, 2022

DOI:

10.3791/64457-v

DOI:

10.3791/64457-v

•

07:12 min

September 07, 2022

•

2175 Views

Adeel Ahmed¹, Indranil M. Joshi¹, Madeleine R. Goulet¹, Justin A. Vidas¹, Ann M. Byerley¹, Mehran Mansouri¹, Steven W. Day¹, Vinay V. Abhyankar¹

¹Department of Biomedical Engineering, Kate Gleason College of Engineering,Rochester Institute of Technology

Summary

This protocol demonstrates the use of a microfluidic channel with changing geometry along the fluid flow direction to generate extensional strain (stretching) to align fibers in a 3D collagen hydrogel (<250 µm in thickness). The resulting alignment extends across several millimeters and is influenced by the extensional strain rate.