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Quantification of Circular RNAs Using Digital Droplet PCR
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JoVE Journal Biology
Quantification of Circular RNAs Using Digital Droplet PCR

Quantification of Circular RNAs Using Digital Droplet PCR

DOI:
10.3791/64464-v

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08:39 min

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September 16, 2022

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, ,
1Institute of Life Sciences, 2School of Biotechnology,KIIT University

Chapters

  • 00:04Introduction
  • 01:02Divergent Primer Design for circRNA and RNA Isolation
  • 03:16cDNA Synthesis
  • 04:09Digital Droplet PCR (dd-PCR) Workflow
  • 07:04Results: Data Analysis Using QuantaSoft Software
  • 08:05Conclusion

Summary

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This protocol describes the detailed method of digital droplet PCR (dd-PCR) for precise quantification of circular RNA (circRNA) levels in cells using divergent primers.

Tags

Digital Droplet PCRDd-PCRAbsolute QuantificationMolecular DiagnosticsCircular RNALow Abundant RNART-PCRRT-qPCRC2C12 MyoblastC2C12 MyotubeRNA IsolationChloroformMagnetic Silica BeadsSpectrophotometer

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