Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / A Triple Primary Cell Culture Model of the Human…
JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content.

JoVE Journal Biochemistry
A Triple Primary Cell Culture Model of the Human Blood-Brain Barrier for Studying Ischemic Stroke In Vitro
Oil and Crystallization Cocktail Well Plate Preparation (Video) | JoVE
Journal (Biochemistry)
Oil and Crystallization Cocktail Well Plate Preparation (Video) | JoVE
Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique
Journal (Biochemistry)
Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique
 

A Triple Primary Cell Culture Model of the Human Blood-Brain Barrier for Studying Ischemic Stroke In Vitro

Article DOI: 10.3791/64469-v • 11:06 min • October 6th, 2022
October 6th, 2022 •
1, 1, 1, 1, 1, 1,2
1Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, 2Institute of Physiotherapy and Health Sciences, The Jerzy Kukuczka Academy of Physical Education

Chapters

Summary
Automatic Translation

Here, we describe the method for establishing a triple cell culture model of the blood-brain barrier based on primary human brain microvascular endothelial cells, astrocytes, and pericytes. This multicellular model is suitable for studies of neurovascular unit dysfunction during ischemic stroke in vitro or for the screening of drug candidates.

Tags

Triple Primary Cell Culture Model Human Blood-brain Barrier Ischemic Stroke In Vitro Study Post-stroke Recovery Molecular And Cellular Changes Novel Targets Therapeutics HBVP T75 Culture Flasks Cell Adhesion Carbon Dioxide Incubator Dulbecco's PBS Trypsin EDTA Solution Pericyte Medium
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter