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JoVE Journal Developmental Biology
Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells에서 망막 오가노이드 생성
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Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells에서 망막 오가노이드 생성

Article DOI: 10.3791/64509-v • 09:47 min • December 9th, 2022
December 9th, 2022 •
*1,2, *1,2, *1,2, 1, 1,3, 1
1Centre for Ocular Regeneration, Prof. Brien Holden Eye Research Centre, LV Prasad Eye Institute, 2Manipal Academy of Higher Education, 3Schepens Eye Research Institute, Massachusetts Eye and Ear, Harvard Medical School
* These authors contributed equally

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이 프로토콜은 hiPSC를 안구 클러스터로 분화하고 부착 및 현탁 배양 시스템을 모두 포함하는 단순화된 배양 조건을 사용하여 신경-망막 오가노이드를 생성하는 효율적인 방법을 설명합니다. RPE 및 각막 상피와 같은 다른 안구 세포 유형도 망막 배양의 성숙한 안구에서 분리할 수 있습니다.

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발달 생물학 제 190 호 줄기 세포 iPSC 망막 분화 안구 원시 망막 오가노이드 망막 색소 상피
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