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Journal / Biochemistry / Live-cell beeldvorming van intacte Ex Vivo Globes …
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Biochemistry

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JoVE Journal Biochemistry
Live-cell beeldvorming van intacte Ex Vivo Globes met behulp van een nieuwe 3D-geprinte houder
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Live-cell beeldvorming van intacte Ex Vivo Globes met behulp van een nieuwe 3D-geprinte houder

Article DOI: 10.3791/64510-v • 05:41 min • October 6th, 2022
October 6th, 2022 •
*1, *2, 2, 3, 2,3
1Department of Pharmacology, School of Medicine, Boston University, 2Department of Biochemistry, School of Medicine, Boston University, 3Department of Ophthalmology, School of Medicine, Boston University
* These authors contributed equally

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Het huidige werk beschrijft een nieuw experimenteel protocol dat een 3D-geprinte houder gebruikt om live cell imaging met hoge resolutie van enucleated globes mogelijk te maken. Via dit protocol kan de cellulaire calciumsignaleringsactiviteit in gewond hoornvliesepitheel van ex vivo globes in realtime worden waargenomen.

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Biochemie nummer 188
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