Ecotoxicological Methodologies to Evaluate Biomarkers at Different Scales in Neotropical Anurans

While the techniques for evaluating biochemical biomarkers are extensively utilized, this protocol present crucial details for standardizing and replicating the methodology in neotropical anurans. The main advantage of this technique is its low cost, replicability, and its application. This technique can be applied to obtain quantitative data to evaluate alterations in the internal melanin.

The comet assay is a sensitive, versatile, and simple technique that allows the determination of genotoxic effects induced by a specific compound. It can be applied to any type of eukaryotic cells to monitor DNA damage, DNA repair, and assess the antioxidant status of cells. To begin, open the software Image-Pro Plus 6.0 and select menu and toolbar as biological.