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Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli
JoVE Journal
Genetics
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JoVE Journal Genetics
Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli

Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli

DOI:
10.3791/64523-v

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06:56 min

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March 24, 2023

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, , , , , ,
1School of Biological Sciences,University of California Irvine, 2Department of Microbiology & Environmental Toxicology,University of California Santa Cruz

Chapters

  • 00:05Introduction
  • 01:15Conjugation
  • 03:16Polymerase Chain Reaction (PCR) and Agarose Gel Electrophoresis
  • 05:10Results: Conjugative Plasmid Detection and Transfer
  • 06:04Conclusion

Summary

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Conjugation mediates horizontal gene transfer by mobilizing plasmid DNA across two different cells, facilitating the spread of beneficial genes. This work describes a widely-used method for the efficient detection of conjugative plasmid transfer, based on the use of differential markers in the conjugative plasmid, donor, and recipient to detect transconjugation.

Tags

Keywords: Horizontal Gene TransferConjugative PlasmidsE. ColiAntibiotic ResistanceEpidemiological SurveillanceMating AssayDonorRecipientSelective Media

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