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Autofluorescentie beeldvorming om de fysiologie van rode algen te evalueren
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Autofluorescentie beeldvorming om de fysiologie van rode algen te evalueren

Article DOI: 10.3791/64533-v • 05:54 min • February 17th, 2023
February 17th, 2023 •
1, 2,3, 4
1Microscopy Core Facility, Technological Research and Scientific Area (ACTI), University of Murcia, 2Confocal Microscopy and Cellular Imaging Unit, Genetic and Molecular Medicine Department, Pediatric Institute for Rare Diseases, Hospital Sant Joan de Déu, 3Research Institute Sant Joan de Déu, Esplugues de Llobregat, Spain 4Algology Laboratory, Biology Faculty, University of Murcia, 4Algology Laboratory, Biology Faculty, University of Murcia

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Het huidige protocol beschrijft stapsgewijze autofluorescentiebeeldvorming en evaluatie van fycobiliproteïneveranderingen in rode algen op basis van spectrale analyse. Dit is een labelvrije en niet-destructieve methode om cellulaire aanpassing aan extreme habitats te evalueren, wanneer alleen schaars materiaal beschikbaar is en cellen langzaam of helemaal niet groeien onder laboratoriumomstandigheden.

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Biologie autofluorescentie fycobilinen chlorofyl confocale laserscanmicroscopie (CLSM) rode algen Chroothece
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