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Chapters
- 00:05Introduction
- 00:31Cell Culture and Growth Curve
- 02:21Determination of Minimal Inhibitory Concentration of Antibiotics
- 04:03Time‐Kill Assay
- 06:24Microfluidic Time‐Lapse Microscopy Imaging
- 09:57Results: Population and Single‐Cell Analysis of Antibiotic Persistence
- 11:47Conclusion
Antibiotic persistence describes the ability of small subpopulations within a sensitive isogenic population to transiently tolerate high doses of bactericidal antibiotics. The present protocol combines approaches to characterize the antibiotic persistence phenotype at the molecular and cellular levels after exposing Escherichia coli to lethal doses of ofloxacin.
