Journal
/
Bioengineering
/
Controlling Particle Fraction in Microporous Annealed Particle Scaffolds for 3D Cell Culture
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Controlling Particle Fraction in Microporous Annealed Particle Scaffolds for 3D Cell Culture

Controlling Particle Fraction in Microporous Annealed Particle Scaffolds for 3D Cell Culture

DOI:
10.3791/64554-v

•

08:11 min

•

October 28, 2022

•

,
1Department of Biomedical Engineering,Duke University, 2Departments of Biomedical Engineering, Neurology, and Dermatology,Duke University

Chapters

  • 00:04Introduction
  • 01:12Drying Hyaluronic Acid (HA)-Norbornene (NB)
  • 02:423D Cell Culture in Microporous Annealed Particle (MAP) Scaffolds
  • 06:03Results: Generating Granular Scaffolds with Controlled Particle Fractions for In Vitro Tissue Engineering
  • 07:32Conclusion

Summary

Automatic Translation

Automatic Translation

Minimizing the variability in the particle fraction within granular scaffolds facilitates reproducible experimentation. This work describes methods for generating granular scaffolds with controlled particle fractions for in vitro tissue engineering applications.

Tags

Particle FractionMicroporous Annealed Particle Scaffolds3D Cell CultureGranular MaterialsMAP ScaffoldsHydrogel ParticlesScaffold PropertiesCell ResponsesWound RepairDrug DeliveryMicrofluidic Microgel GenerationHyaluronic AcidNorborneneMicrogelsLyophilizationPolydimethylsiloxane

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Preparation of 3D Fibrin Scaffolds for Stem Cell Culture Applications

Micro-particle Image Velocimetry for Velocity Profile Measurements of Micro Blood Flows

Self-reporting Scaffolds for 3-Dimensional Cell Culture

Longitudinal Measurement of Extracellular Matrix Rigidity in 3D Tumor Models Using Particle-tracking Microrheology

3D Hydrogel Scaffolds for Articular Chondrocyte Culture and Cartilage Generation

In Situ Mapping of the Mechanical Properties of Biofilms by Particle-tracking Microrheology

Tunable Hydrogels from Pulmonary Extracellular Matrix for 3D Cell Culture

Synthesis of Biocompatible Liquid Crystal Elastomer Foams as Cell Scaffolds for 3D Spatial Cell Cultures

A Protocol for Real-time 3D Single Particle Tracking

Meso-Scale Particle Image Velocimetry Studies of Neurovascular Flows In Vitro

Read Article