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Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue
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Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue

Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue

DOI:
10.3791/64614-v

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07:35 min

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October 06, 2022

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, , ,
1Depto. Biología Celular, Genética y Fisiología, Instituto de Hortofruticultura Subtropical y Mediterránea, Campus de Teatinos,Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC)

Chapters

  • 00:05Introduction
  • 00:54Plant Preparation
  • 02:14Inoculation of Arabidopsis and Bean Plants
  • 03:53Extraction of Bacteria from the Apoplast
  • 04:55Single-Cell Analysis of the Apoplast-Extracted Bacteria
  • 06:14Results: Analysis of the Apoplast-Extracted Bacteria Obtained from Arabidopsis or Bean Leaves After Bacterial Inoculation
  • 06:57Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol describes a method that allows single-cell gene expression analysis on Pseudomonas syringae populations grown within the plant apoplast.

Tags

Keywords: Single-cell AnalysisPseudomonas SyringaeApoplastPlant InoculationBacterial ExtractionFlow CytometryPlant Pathogenic BacteriaBeneficial Endocytic BacteriaArabidopsisPhaseolus VulgarisLB PlateOptical Density

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