The present protocol describes a method that allows single-cell gene expression analysis on Pseudomonas syringae populations grown within the plant apoplast.
Rufián, J. S., López-Pagán, N., Ruiz-Albert, J., Beuzón, C. R. Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue. J. Vis. Exp. (188), e64614, doi:10.3791/64614 (2022).