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A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials

A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials

DOI:
10.3791/64641-v

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06:18 min

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March 03, 2023

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, ,
1Harris Orthopaedics Laboratory,Massachusetts General Hospital, 2Department of Orthopaedic Surgery,Harvard Medical School

Chapters

  • 00:04Introduction
  • 01:01Time‐Dependent Antibacterial Activity Study Setup
  • 03:05Quantification of Adherent Bacteria
  • 03:42Results: Ultrahigh Molecular Weight Polyethylene (UHMWPE) Implant‐Induced Antibiotic Activity
  • 05:25Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to evaluate the antibacterial efficacy of an antibiotic-eluting polymer to simulate prophylactic clinical application by using a commercially available real-time ATP-based luminescent microbial viability assay. This method enables the monitoring of the longitudinal activity of drug-eluting materials and can be widely adapted to test anti-microbial drug delivery platforms.

Tags

Keywords: Antibacterial ActivityDrug-eluting MaterialsPolymeric DevicePeriprosthetic Joint InfectionsReal-time Microbial Viability AssayColony Forming UnitsLongitudinal Antibacterial ActivityUHMWPEDrug-eluting DeviceAntibacterial Efficacy

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