Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome

Bilin Liu; Anqi Li; Yuan Qin; Lei Chen; Meng Gao; Guohua Gong

doi:10.3791/64647

Journal

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Biology

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Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome

JoVE Journal
Biology
Author Produced

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JoVE Journal Biology

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome

4,351 Views

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07:56 min

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November 30, 2022

DOI:

10.3791/64647-v

DOI:

10.3791/64647-v

•

07:56 min

November 30, 2022

•

4281 Views

Bilin Liu^1,2, Anqi Li¹, Yuan Qin³, Lei Chen¹, Meng Gao¹, Guohua Gong^1,2

¹Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology,Tongji University ²Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences,Wenzhou Medical University ³Department of Pharmacy, Shanghai East Hospital,Tongji University

Summary

Mitophagy is the primary mechanism of mitochondrial quality control. However, the evaluation of mitophagy in vivo is hindered by the lack of reliable quantitative assays. Presented here is a protocol for the observation of mitophagy in living cells using a cell-permeant green-fluorescent mitochondria dye and a red-fluorescent lysosome dye.