Mitophagy is the primary mechanism of mitochondrial quality control. However, the evaluation of mitophagy in vivo is hindered by the lack of reliable quantitative assays. Presented here is a protocol for the observation of mitophagy in living cells using a cell-permeant green-fluorescent mitochondria dye and a red-fluorescent lysosome dye.
Liu, B., Li, A., Qin, Y., Chen, L., Gao, M., Gong, G. Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome. J. Vis. Exp. (189), e64647, doi:10.3791/64647 (2022).