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Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome
 

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome

Article DOI: 10.3791/64647-v
November 30th, 2022

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Summary November 30th, 2022

Mitophagy is the primary mechanism of mitochondrial quality control. However, the evaluation of mitophagy in vivo is hindered by the lack of reliable quantitative assays. Presented here is a protocol for the observation of mitophagy in living cells using a cell-permeant green-fluorescent mitochondria dye and a red-fluorescent lysosome dye.

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