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Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome
Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome
JoVE Journal
Biology
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JoVE Journal Biology
Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome

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07:56 min

November 30, 2022

DOI:

07:56 min
November 30, 2022

4281 Views
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Summary

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Mitophagy is the primary mechanism of mitochondrial quality control. However, the evaluation of mitophagy in vivo is hindered by the lack of reliable quantitative assays. Presented here is a protocol for the observation of mitophagy in living cells using a cell-permeant green-fluorescent mitochondria dye and a red-fluorescent lysosome dye.

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