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Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing
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Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing

Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing

DOI:
10.3791/64688-v

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05:34 min

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February 10, 2023

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, , , , , ,
1State Key Laboratory of Marine Resource Utilization in South China Sea,Hainan University, 2Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, College of Fishery,Guangdong Ocean University, 3Hainan Provincial Key Laboratory for Tropical Hydrobiology and Biotechnology, College of Marine Science,Hainan University, 4Department of Animal Wealth Development, Faculty of Veterinary Medicine,Suez Canal University

Chapters

  • 00:05Introduction
  • 00:42Fish, Reagent, and Equipment Preparation
  • 01:18Tissue Dissection and Cell Dissociation
  • 03:50Staining and Microscopic Examination
  • 04:16Results: Effect of Enzymes on Tissue Dissociation
  • 04:52Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we demonstrate the preparation of a high-quality single-cell suspension of tilapia intestine for single-cell sequencing.

Tags

Keywords: Single-cell SuspensionNile TilapiaIntestineSingle-cell SequencingEnzymatic Cell DissociationCollagenase DispasePBSFBSIntestinal ContentsMucusBSA-DPBSRNA InhibitorCell Strainer

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