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Human Liver Spheroids from Peripheral Blood for Liver Disease Studies
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Bioengineering
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JoVE Journal Bioengineering
Human Liver Spheroids from Peripheral Blood for Liver Disease Studies

Human Liver Spheroids from Peripheral Blood for Liver Disease Studies

DOI:
10.3791/64703-v

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09:51 min

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January 27, 2023

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, , ,
1ACA CELL Biotech GmbH, 2Departamento Ciencias Biomédicas,Universidad Cardenal Herrera-CEU

Chapters

  • 00:05Introduction
  • 00:58Preparation of Hepatocyte Differentiation Media
  • 01:27Culturing Hepatic Cells Differentiated from Blood-Derived Pluripotent Stem Cells (BD-PSCs)
  • 02:143D Spheroid Hepatic Differentiation
  • 03:34Immunofluorescence Analysis of Newly Generated 2D Liver Cell Cultures
  • 04:59Live Staining of Newly Formed Liver Spheroids
  • 06:01Examination of Spheroids Using a Fluorescence Microscope
  • 07:32Results: Differentiation of Human BD-PSCs into Endoderm/Hepatic Progenitor Cells and Hepatocytes
  • 09:11Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a non-genetic method to generate human autologous liver spheroids using mononuclear cells isolated from steady-state peripheral blood.

Tags

Keywords: Liver SpheroidsPeripheral BloodAutologous SourcesToxicologyCancerDrug DiscoveryBDPC-derived HepatocytesPrimary Human HepatocytesRegenerative MedicineBlood-derived Pluripotent Stem CellsEndodermal DifferentiationHepatocyte MaturationLow Attachment PlatesSpheroid Formation

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