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A Human Bone Marrow 3D Model to Investigate the Dynamics and Interactions Between Resident Cells in Physiological or Tumoral Contexts
JoVE Journal
Biology
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JoVE Journal Biology
A Human Bone Marrow 3D Model to Investigate the Dynamics and Interactions Between Resident Cells in Physiological or Tumoral Contexts

A Human Bone Marrow 3D Model to Investigate the Dynamics and Interactions Between Resident Cells in Physiological or Tumoral Contexts

DOI:
10.3791/64736-v

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09:07 min

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December 16, 2022

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, , , , , , , , , ,
1Centre de Recherche en Cancérologie de Lyon, 2Centre de Recherche en Cancérologie de Lyon, 3Université de Lyon, 4Centre Léon Bérard, 5Department of Experimental and Clinical Biomedical Sciences Mario Serio,Università degli Studi di Firenze

Chapters

  • 00:04introduction
  • 00:45Bead Preparation and Sterilization
  • 01:133D Insert Preparation Under a Sterile Hood
  • 02:04Cell Line Harvesting and 3D Maintenance
  • 03:32Addition of Cells of Interest into the System
  • 04:073D Bone Marrow-Like Experimental Results
  • 07:50Results: In Vitro 3D Human Bone Marrow-Like Microenvironment for Understanding the Resident Cell Interactions
  • 08:33Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe an easy-to-implement, standardized, microphysiological system that reflects the complexity of the human bone marrow's in vivo structure, providing a pertinent model to finely study a broad range of normal and pathological events.

Tags

Keywords: 3D ModelBone MarrowResident CellsPhysiological ContextTumoral ContextStandardized SystemHuman Cell LinesPrimary Cell PopulationsBone Marrow-like StructureCell HarvestIn Situ Cell Localization3D Structure DissociationCellular ComponentMolecular StudiesFunctional StudiesBCP BeadsPolycarbonate MembraneCell Culture InsertsHMEC-1 CellsHS27A CellsVascular CompartmentMesenchymal Stromal Cell CompartmentOsteoblast DifferentiationCell Suspension

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